biology/pooler: Optimise DNA sequencing primer-set combinations

Optimise combinations of primers and minimise the formation of dimers in
multiplexed PCR.

Primer Pooler can:

• Check through each proposed pool for combinations that are likely to form dimers

• Automatically move prospective amplicons between proposed pools to reduce dimer formation

• Automatically search the genome sequence to find which amplicons overlap, and place their corresponding primers in separate pools

• Optionally keep pool sizes within a specified range

• Handle thousands of primers without being slow (useful for high-throughput sequencing applications)

• Do all of the above with degenerate primers too.

WWW: http://ssb22.user.srcf.net/pooler/

PR: ports/251065
Submitted by: Silas S. Brown <silas-freebsd@flatline.org.uk>