Index: head/biology/p5-TrimGalore/Makefile
===================================================================
--- head/biology/p5-TrimGalore/Makefile	(revision 496775)
+++ head/biology/p5-TrimGalore/Makefile	(revision 496776)
@@ -1,31 +1,31 @@
 # $FreeBSD$
 
 PORTNAME=	TrimGalore
 DISTVERSION=	0.4.5
 CATEGORIES=	biology perl5
 PKGNAMEPREFIX=	p5-
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Wrapper around Cutadapt and FastQC for adapter and quality trimming
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 RUN_DEPENDS=	${PYTHON_PKGNAMEPREFIX}cutadapt>0:biology/py-cutadapt@${PY_FLAVOR} \
 		fastqc>0:biology/fastqc
 
 NO_ARCH=	yes
 NO_BUILD=	yes
 
-USES=		perl5 python:env shebangfix
+USES=		perl5 python:3.4+,env shebangfix
 SHEBANG_FILES=	trim_galore
 USE_PERL5=	run
 
 USE_GITHUB=	yes
 GH_ACCOUNT=	FelixKrueger
 
 do-install:
 	${INSTALL_SCRIPT} ${WRKSRC}/trim_galore ${STAGEDIR}${PREFIX}/bin
 	cd ${WRKSRC} && ${COPYTREE_SHARE} test_files ${STAGEDIR}${DATADIR}
 
 .include <bsd.port.mk>
Index: head/biology/py-cutadapt/Makefile
===================================================================
--- head/biology/py-cutadapt/Makefile	(revision 496775)
+++ head/biology/py-cutadapt/Makefile	(revision 496776)
@@ -1,24 +1,24 @@
 # $FreeBSD$
 
 PORTNAME=	cutadapt
 PORTVERSION=	1.18
 CATEGORIES=	biology python
 MASTER_SITES=	CHEESESHOP
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Trim adapters from high-throughput sequencing reads
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 RUN_DEPENDS=	${PYTHON_PKGNAMEPREFIX}xopen>=0.3.2:archivers/py-xopen@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}bz2file>=0.98:archivers/py-bz2file@${PY_FLAVOR}
 
-USES=		python
+USES=		python:3.4+
 USE_PYTHON=	autoplist concurrent distutils
 
 post-install:
 	@${STRIP_CMD} ${STAGEDIR}${PYTHON_SITELIBDIR}/cutadapt/*.so
 
 .include <bsd.port.mk>