diff --git a/biology/vsearch/Makefile b/biology/vsearch/Makefile
index 7a264f379a41..f517400f6e34 100644
--- a/biology/vsearch/Makefile
+++ b/biology/vsearch/Makefile
@@ -1,29 +1,29 @@
 PORTNAME=		vsearch
 DISTVERSIONPREFIX=	v
-DISTVERSION=		2.19.0
+DISTVERSION=		2.21.1
 CATEGORIES=		biology
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Versatile open-source tool for metagenomics
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/LICENSE.txt
 
 ONLY_FOR_ARCHS=		aarch64 amd64 powerpc64le
 ONLY_FOR_ARCHS_REASON=	Explicitly enumerated by upstream developers
 
 LIB_DEPENDS=	libsysinfo.so:devel/libsysinfo
 
 USES=		autoreconf ghostscript:build gmake localbase
 USE_GITHUB=	yes
 
 GH_ACCOUNT=	torognes
 GNU_CONFIGURE=	yes
 
 PLIST_FILES=	bin/vsearch man/man1/vsearch.1.gz
 PORTDOCS=	*
 
 OPTIONS_DEFINE=	DOCS
 OPTIONS_SUB=	yes
 
 .include <bsd.port.mk>
diff --git a/biology/vsearch/distinfo b/biology/vsearch/distinfo
index ff23577ebb73..85343620dba7 100644
--- a/biology/vsearch/distinfo
+++ b/biology/vsearch/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1641573705
-SHA256 (torognes-vsearch-v2.19.0_GH0.tar.gz) = 74bc30786512d07e49836320af1e745ef527c4472317621d3bd0b50cbc218736
-SIZE (torognes-vsearch-v2.19.0_GH0.tar.gz) = 253686
+TIMESTAMP = 1642976868
+SHA256 (torognes-vsearch-v2.21.1_GH0.tar.gz) = 0c5f4c981bc8ecbf23ee618d1b45667f81ae9845d64b9d6a310edbd196c84638
+SIZE (torognes-vsearch-v2.21.1_GH0.tar.gz) = 257434