diff --git a/biology/seqtk/Makefile b/biology/seqtk/Makefile
index 6a779dfd93b4..0a7e6ac9682b 100644
--- a/biology/seqtk/Makefile
+++ b/biology/seqtk/Makefile
@@ -1,17 +1,17 @@
 PORTNAME=		seqtk
 DISTVERSIONPREFIX=	v
-DISTVERSION=		1.3
+DISTVERSION=		1.4
 CATEGORIES=		biology
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Tool for processing sequences in FASTA/FASTQ format
 WWW=		https://github.com/lh3/seqtk
 
 LICENSE=	GPLv2
 
 USE_GITHUB=	yes
 GH_ACCOUNT=	lh3
 
 PLIST_FILES=	bin/seqtk
 
 .include <bsd.port.mk>
diff --git a/biology/seqtk/distinfo b/biology/seqtk/distinfo
index f44856371472..b790163bdb01 100644
--- a/biology/seqtk/distinfo
+++ b/biology/seqtk/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1639855187
-SHA256 (lh3-seqtk-v1.3_GH0.tar.gz) = 5a1687d65690f2f7fa3f998d47c3c5037e792f17ce119dab52fff3cfdca1e563
-SIZE (lh3-seqtk-v1.3_GH0.tar.gz) = 21196
+TIMESTAMP = 1684848703
+SHA256 (lh3-seqtk-v1.4_GH0.tar.gz) = d124604ec24f29ed14ce127426ab90e0f3a2c0280c80d1a3ff8b1c09feede19c
+SIZE (lh3-seqtk-v1.4_GH0.tar.gz) = 23748