diff --git a/biology/hyphy/Makefile b/biology/hyphy/Makefile
index b18cba727f82..38455c99d569 100644
--- a/biology/hyphy/Makefile
+++ b/biology/hyphy/Makefile
@@ -1,28 +1,29 @@
 PORTNAME=	hyphy
-DISTVERSION=	2.5.64
+DISTVERSION=	2.5.65
 CATEGORIES=	biology
 
 MAINTAINER=	jrm@FreeBSD.org
 COMMENT=	Hypothesis testing using Phylogenies
 WWW=		https://www.hyphy.org/ \
 		https://github.com/veg/hyphy/
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 ONLY_FOR_ARCHS=	aarch64 amd64 i386 powerpc powerpc64 powerpc64le
 
 LIB_DEPENDS=	libcurl.so:ftp/curl
 TEST_DEPENDS=	bash:shells/bash
 
 USES=		cmake:insource,testing localbase:ldflags # tests fail in outsource build, see https://github.com/veg/hyphy/issues/1528
 
 USE_GITHUB=	yes
 GH_ACCOUNT=	veg
+GH_TAGNAME=	840701205917b27f1074d6c41fb2bfdece62431a
 
 OPTIONS_DEFINE=	AVX
 AVX_DESC=	Advanced vector extensions support
 
-AVX_CMAKE_OFF=	-DNOAVX:BOOL=ON
+AVX_CMAKE_BOOL_OFF=	NOAVX
 
 .include <bsd.port.mk>
diff --git a/biology/hyphy/distinfo b/biology/hyphy/distinfo
index ffa4bb48bcc2..1a7bec0e71c6 100644
--- a/biology/hyphy/distinfo
+++ b/biology/hyphy/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1732814990
-SHA256 (veg-hyphy-2.5.64_GH0.tar.gz) = 7ba1aaf82c8d876d36dc9deb63da8a5da604917fe1628cef444af0207579aea6
-SIZE (veg-hyphy-2.5.64_GH0.tar.gz) = 5613927
+TIMESTAMP = 1738096315
+SHA256 (veg-hyphy-2.5.65-840701205917b27f1074d6c41fb2bfdece62431a_GH0.tar.gz) = afc306e2fbebce65f2e21b41888fc181f854f74bb6ab1ae1c928a020243f7a24
+SIZE (veg-hyphy-2.5.65-840701205917b27f1074d6c41fb2bfdece62431a_GH0.tar.gz) = 5616313