diff --git a/biology/seqkit/Makefile b/biology/seqkit/Makefile
index 8daca8887e77..f64ae1af9267 100644
--- a/biology/seqkit/Makefile
+++ b/biology/seqkit/Makefile
@@ -1,25 +1,24 @@
 PORTNAME=	seqkit
 DISTVERSIONPREFIX=	v
-DISTVERSION=	2.3.1
-PORTREVISION=	6
+DISTVERSION=	2.4.0
 CATEGORIES=	biology
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Cross-platform and ultrafast toolkit for FASTA/Q file manipulation
 WWW=		https://bioinf.shenwei.me/seqkit/
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 USES=		go:modules
 
 GO_MODULE=	github.com/shenwei356/seqkit/v2
 GO_TARGET=	./${PORTNAME}
 
 PLIST_FILES=	bin/${PORTNAME}
 
 do-test: # from https://bioinf.shenwei.me/seqkit/benchmark/
 	@cd ${WRKSRC}/tests && \
 		${GO_WRKDIR_BIN}/seqkit stat *.fa
 
 .include <bsd.port.mk>
diff --git a/biology/seqkit/distinfo b/biology/seqkit/distinfo
index 9cb658d54392..75736a5f4b6e 100644
--- a/biology/seqkit/distinfo
+++ b/biology/seqkit/distinfo
@@ -1,5 +1,5 @@
-TIMESTAMP = 1664069346
-SHA256 (go/biology_seqkit/seqkit-v2.3.1/v2.3.1.mod) = fdf7d84119bb0ea9f03f3b68cb63dfc055e9d4187609e0eb2554bbd8b39600f0
-SIZE (go/biology_seqkit/seqkit-v2.3.1/v2.3.1.mod) = 2322
-SHA256 (go/biology_seqkit/seqkit-v2.3.1/v2.3.1.zip) = 68a5586bc6f240dd419a5e6d7fa73e13c51dfdaba1bd659510fa2cfa79a5bdcb
-SIZE (go/biology_seqkit/seqkit-v2.3.1/v2.3.1.zip) = 33892763
+TIMESTAMP = 1679088879
+SHA256 (go/biology_seqkit/seqkit-v2.4.0/v2.4.0.mod) = b2e9bfdf3899c8c4f9a736e0a18599ec36784db188fbce8c4dc1e578a7bfe2f8
+SIZE (go/biology_seqkit/seqkit-v2.4.0/v2.4.0.mod) = 2391
+SHA256 (go/biology_seqkit/seqkit-v2.4.0/v2.4.0.zip) = 31ac20c6354ff852b9cc3c5506e48154cbe76605e3f8961a7043f76533cee231
+SIZE (go/biology_seqkit/seqkit-v2.4.0/v2.4.0.zip) = 33905729