diff --git a/biology/vsearch/Makefile b/biology/vsearch/Makefile
index 79e8b07cc801..b5fd0b6079e9 100644
--- a/biology/vsearch/Makefile
+++ b/biology/vsearch/Makefile
@@ -1,29 +1,28 @@
 PORTNAME=		vsearch
 DISTVERSIONPREFIX=	v
-DISTVERSION=		2.24.0
-PORTREVISION=		1
+DISTVERSION=		2.25.0
 CATEGORIES=		biology
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Versatile open-source tool for metagenomics
 WWW=		https://github.com/torognes/vsearch
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/LICENSE.txt
 
 ONLY_FOR_ARCHS=		aarch64 amd64 powerpc64le
 ONLY_FOR_ARCHS_REASON=	Explicitly enumerated by upstream developers
 
 USES=		autoreconf ghostscript:build gmake localbase
 USE_GITHUB=	yes
 
 GH_ACCOUNT=	torognes
 GNU_CONFIGURE=	yes
 
 PLIST_FILES=	bin/vsearch man/man1/vsearch.1.gz
 PORTDOCS=	*
 
 OPTIONS_DEFINE=	DOCS
 OPTIONS_SUB=	yes
 
 .include <bsd.port.mk>
diff --git a/biology/vsearch/distinfo b/biology/vsearch/distinfo
index 8cf35e86430e..b7b1fad6da9b 100644
--- a/biology/vsearch/distinfo
+++ b/biology/vsearch/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1698407468
-SHA256 (torognes-vsearch-v2.24.0_GH0.tar.gz) = 036c3a5c7827958446e56155d750d22d03d460a53237c37fa6541208dc0bdca1
-SIZE (torognes-vsearch-v2.24.0_GH0.tar.gz) = 267096
+TIMESTAMP = 1699797850
+SHA256 (torognes-vsearch-v2.25.0_GH0.tar.gz) = b7e25638924e56591d7c6ac3a959795000439937f5b566be7f06457bb31f91a9
+SIZE (torognes-vsearch-v2.25.0_GH0.tar.gz) = 267819