diff --git a/biology/py-pysam/Makefile b/biology/py-pysam/Makefile
index 10e1d55b786e..800bef74b901 100644
--- a/biology/py-pysam/Makefile
+++ b/biology/py-pysam/Makefile
@@ -1,28 +1,27 @@
 PORTNAME=	pysam
-DISTVERSION=	0.20.0
-PORTREVISION=	1
+DISTVERSION=	0.21.0
 CATEGORIES=	biology python
 MASTER_SITES=	PYPI
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Python module for reading, manipulating and writing genomic data sets
 WWW=		https://github.com/pysam-developers/pysam
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/COPYING
 
 LIB_DEPENDS=	libhts.so:biology/htslib
 
 USES=		gmake python:3.6-3.9
-USE_PYTHON=	autoplist distutils cython cython_run pytest
+USE_PYTHON=	distutils autoplist cython cython_run
 
 BINARY_ALIAS=	make=${GMAKE}
 MAKE_ENV=	HTSLIB_MODE=external \
 		HTSLIB_LIBRARY_DIR=${LOCALBASE}/lib \
 		HTSLIB_INCLUDE_DIR=${LOCALBASE}/include
 
 post-install:
 	@${STRIP_CMD} ${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}/pysam/lib*${PYTHON_EXT_SUFFIX}.so
 
 .include <bsd.port.mk>
diff --git a/biology/py-pysam/distinfo b/biology/py-pysam/distinfo
index ff34934adf54..1c9903177cca 100644
--- a/biology/py-pysam/distinfo
+++ b/biology/py-pysam/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1668031193
-SHA256 (pysam-0.20.0.tar.gz) = 7cc250148ba0ffc9bdc38db6988b91e13b75db0d11c18cf1336467d1c97dd312
-SIZE (pysam-0.20.0.tar.gz) = 3958505
+TIMESTAMP = 1686950070
+SHA256 (pysam-0.21.0.tar.gz) = 5c9645ddd87668e36ff0a1966391e26f9c403bf85b1bc06c53fe2fcd592da2ce
+SIZE (pysam-0.21.0.tar.gz) = 4052537