diff --git a/biology/vsearch/Makefile b/biology/vsearch/Makefile
index 4937c94ff19b..adef9781e567 100644
--- a/biology/vsearch/Makefile
+++ b/biology/vsearch/Makefile
@@ -1,29 +1,29 @@
 PORTNAME=		vsearch
 DISTVERSIONPREFIX=	v
-DISTVERSION=		2.29.0
+DISTVERSION=		2.29.1
 CATEGORIES=		biology
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Versatile open-source tool for metagenomics
 WWW=		https://github.com/torognes/vsearch
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/LICENSE.txt
 
 ONLY_FOR_ARCHS=		aarch64 amd64 powerpc64le
 ONLY_FOR_ARCHS_REASON=	Explicitly enumerated by upstream developers
 
 USES=		autoreconf ghostscript:build gmake localbase
 USE_GITHUB=	yes
 
 GH_ACCOUNT=	torognes
 GNU_CONFIGURE=	yes
 GNU_CONFIGURE_MANPREFIX=	${PREFIX}/share
 
 PLIST_FILES=	bin/vsearch share/man/man1/vsearch.1.gz
 PORTDOCS=	*
 
 OPTIONS_DEFINE=	DOCS
 OPTIONS_SUB=	yes
 
 .include <bsd.port.mk>
diff --git a/biology/vsearch/distinfo b/biology/vsearch/distinfo
index 55cfb1fec6ff..198bab2f27d6 100644
--- a/biology/vsearch/distinfo
+++ b/biology/vsearch/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1729339584
-SHA256 (torognes-vsearch-v2.29.0_GH0.tar.gz) = 2c5bd0d9b3c2ec8eecd1af06ae11611138d87fdfecfa423ae791d52dccd27e63
-SIZE (torognes-vsearch-v2.29.0_GH0.tar.gz) = 283459
+TIMESTAMP = 1730060326
+SHA256 (torognes-vsearch-v2.29.1_GH0.tar.gz) = a36f175c4eddf1c7ce7030561de52c20d36ddc5ed1f498ee4853c6808f7375bb
+SIZE (torognes-vsearch-v2.29.1_GH0.tar.gz) = 283766