diff --git a/biology/seqkit/Makefile b/biology/seqkit/Makefile
index f1490e748627..fa928d742fd1 100644
--- a/biology/seqkit/Makefile
+++ b/biology/seqkit/Makefile
@@ -1,24 +1,23 @@
 PORTNAME=	seqkit
 DISTVERSIONPREFIX=	v
-DISTVERSION=	2.1.0
-PORTREVISION=	1
+DISTVERSION=	2.2.0
 CATEGORIES=	biology
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Cross-platform and ultrafast toolkit for FASTA/Q file manipulation
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 USES=		go:modules
 
 GO_MODULE=	github.com/shenwei356/seqkit/v2
 GO_TARGET=	./${PORTNAME}
 
 PLIST_FILES=	bin/${PORTNAME}
 
 do-test: # from https://bioinf.shenwei.me/seqkit/benchmark/
 	@cd ${WRKSRC}/tests && \
 		${GO_WRKDIR_BIN}/seqkit stat *.fa
 
 .include <bsd.port.mk>
diff --git a/biology/seqkit/distinfo b/biology/seqkit/distinfo
index 9e479c85af14..3f0580f22468 100644
--- a/biology/seqkit/distinfo
+++ b/biology/seqkit/distinfo
@@ -1,5 +1,5 @@
-TIMESTAMP = 1637087450
-SHA256 (go/biology_seqkit/seqkit-v2.1.0/v2.1.0.mod) = d68685581f3010278f064a290097e2e62386d1ef544951e37c65bf38092280f5
-SIZE (go/biology_seqkit/seqkit-v2.1.0/v2.1.0.mod) = 2053
-SHA256 (go/biology_seqkit/seqkit-v2.1.0/v2.1.0.zip) = d55d1ee9dc6bf5661ebe19f3acc9ba8d389ef2de47b060a91f29bc5433b2adb4
-SIZE (go/biology_seqkit/seqkit-v2.1.0/v2.1.0.zip) = 31850110
+TIMESTAMP = 1650426203
+SHA256 (go/biology_seqkit/seqkit-v2.2.0/v2.2.0.mod) = 700478329f988834e8ec39b5c2b91d52728d1279e1d84dd1fcd0670647653fa8
+SIZE (go/biology_seqkit/seqkit-v2.2.0/v2.2.0.mod) = 2321
+SHA256 (go/biology_seqkit/seqkit-v2.2.0/v2.2.0.zip) = 6bc951e10393c9c8a0c771bba96227f78e50b07b7a93f61bba534f9a35aa8cbc
+SIZE (go/biology_seqkit/seqkit-v2.2.0/v2.2.0.zip) = 33907530