diff --git a/biology/py-pybigwig/Makefile b/biology/py-pybigwig/Makefile
index c54fba8c9e9c..131ba5e45ca1 100644
--- a/biology/py-pybigwig/Makefile
+++ b/biology/py-pybigwig/Makefile
@@ -1,22 +1,22 @@
 PORTNAME=	pyBigWig
-DISTVERSION=	0.3.20
+DISTVERSION=	0.3.21
 CATEGORIES=	biology python
 MASTER_SITES=	PYPI
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Python access to bigWig files using libBigWig
 WWW=		https://pypi.org/project/pyBigWig/
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE.txt
 
 LIB_DEPENDS=	libcurl.so:ftp/curl
 
 USES=		localbase python
 USE_PYTHON=	autoplist distutils
 
 post-install:
 	@${STRIP_CMD} ${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}/pyBigWig*.so
 
 .include <bsd.port.mk>
diff --git a/biology/py-pybigwig/distinfo b/biology/py-pybigwig/distinfo
index 49e194311f81..4d8222cfc082 100644
--- a/biology/py-pybigwig/distinfo
+++ b/biology/py-pybigwig/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1678281929
-SHA256 (pyBigWig-0.3.20.tar.gz) = ba63ffb302647a6bcda75187a9d8576bb249e37ae6e83379ddb31e22cd7a8c0b
-SIZE (pyBigWig-0.3.20.tar.gz) = 65091
+TIMESTAMP = 1680381155
+SHA256 (pyBigWig-0.3.21.tar.gz) = 5bff713fe3f8840e0fbe48cf8c8fe9d92c8af8ba107da0b845076164445d076e
+SIZE (pyBigWig-0.3.21.tar.gz) = 73827