diff --git a/libexec/rc/rc.conf b/libexec/rc/rc.conf
--- a/libexec/rc/rc.conf
+++ b/libexec/rc/rc.conf
@@ -69,6 +69,8 @@
 zfs_enable="NO"		# Set to YES to automatically mount ZFS file systems
 zfskeys_enable="NO"	# Set YES to autoload ZFS encryption keys
 zfs_bootonce_activate="NO" # Set YES to make successful bootonce BE permanent
+zpool_reguid=""		# Set to zpools for which the GUID should be replaced
+			# upon first boot.
 
 # ZFSD support
 zfsd_enable="NO"	# Set to YES to automatically start the ZFS fault
diff --git a/libexec/rc/rc.d/Makefile b/libexec/rc/rc.d/Makefile
--- a/libexec/rc/rc.d/Makefile
+++ b/libexec/rc/rc.d/Makefile
@@ -326,6 +326,7 @@
 ZFS+=		zfsd
 ZFS+=		zfskeys
 ZFS+=		zpool
+ZFS+=		zpoolreguid
 ZFS+=		zvol
 ZFSPACKAGE=	rc
 .endif
diff --git a/libexec/rc/rc.d/zpoolreguid b/libexec/rc/rc.d/zpoolreguid
new file mode 100755
--- /dev/null
+++ b/libexec/rc/rc.d/zpoolreguid
@@ -0,0 +1,25 @@
+#!/bin/sh
+
+# PROVIDE: zpoolreguid
+# REQUIRE: zpool
+# BEFORE: mountcritlocal
+# KEYWORD: firstboot nojail
+
+. /etc/rc.subr
+
+name="zpoolreguid"
+desc="Generate a new zpool GUID"
+rcvar="zfs_enable"
+start_cmd="zpoolreguid_start"
+
+zpoolreguid_start()
+{
+	local pool
+
+	for pool in "${zpool_reguid}"; do
+		zpool reguid $pool
+	done
+}
+
+load_rc_config $name
+run_rc_command "$1"
diff --git a/share/man/man5/rc.conf.5 b/share/man/man5/rc.conf.5
--- a/share/man/man5/rc.conf.5
+++ b/share/man/man5/rc.conf.5
@@ -24,7 +24,7 @@
 .\"
 .\" $FreeBSD$
 .\"
-.Dd March 3, 2022
+.Dd May 26, 2022
 .Dt RC.CONF 5
 .Os
 .Sh NAME
@@ -2067,6 +2067,12 @@
 .Pa /etc/rc.d/zfs
 will attempt to automatically mount ZFS file systems and initialize ZFS volumes
 (ZVOLs).
+.It Va zpool_reguid
+.Pq Vt str
+A space-separated list of ZFS pool names for which new pool GUIDs should be
+assigned upon first boot.
+This is useful when using a ZFS pool copied from a template, such as a virtual
+machine image.
 .It Va gptboot_enable
 .Pq Vt bool
 If set to